2d And 3d Cell Culture

Recently three dimensional 3d cell cultures have received remarkable attention in studies such as drug discovery and development.

2d and 3d cell culture. Petersen et al 1992. Cellular events in 3d culture resemble physiological conditions closely and have the following distinct advantages over the 2d culture conditions. The primary changes to cells in 3d are rather obvious shape motility and polarity are all affected but can have important secondary less obvious tissue specific consequences. 3d cell culture a new dimension.

The key difference between 2d and 3d cell culture is that the 2d cell culture uses an artificial flat surface typically a petri dish or a cell culture plate while the 3d cell culture uses a substrate that mimics the extracellular matrix of that particular cell type. Simple inexpensive and reproducible traditional or 2d cell culture is the mainstay of biological research. However 2d cell cultures poorly imitate the conditions in vivo. Meanwhile 3d cell culture systems feature increased complexity for increased faithfulness to the in vivo environment.

A basic method known as hanging drop was tested by ross granville 1870 1959 and led to advances in a number of areas of biology including oncology and genetics. Advantages of 3d cell culture. Although it is more laborious and technically challenging to establish a 3d culture model compared with 2d cultures the 3d culture system is biophysically and biochemically much more similar to in vivo tissues organs with the feasibility to mimic in vivo microenvironment as well as cell cell and cell matrix interactions. Stem cells grown in 3d exhibit significantly higher differentiation potential 15.

Approximately 300 spheroids are usually cultured per bioreact. One study compared a 2d monolayer cell culture to a 3d cell culture in which 24 malignant and non malignant breast cell lines were cultured generated by lrecm barcellos hoff et al 1989. Unlike 2d environments a 3d cell culture allows cells in vitro to grow in all directions similar to how they would in vivo. A 3d cell culture is an artificially created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions.

3d cell culture has technically been around for a long time. Two dimensional 2d cell cultures have been used since 1900s and are still a dominant method in many biological studies. Easier cell observation and measurement 2d cell cultures are typically easier to analyze than some 3d cell culture systems. Please use one of the following formats to cite this article in your essay paper or report.

3d cell culture techniques the reason for the growth in 3d cell culture is it s simply a better way of representing human tissue outside the body. These three dimensional cultures are usually grown in bioreactors small capsules in which the cells can grow into spheroids or 3d cell colonies. These are just some of the reasons why a 3d environment for culture has been gaining traction in recent years.